Jundishapur Journal of Microbiology

Published by: Kowsar

FLASH-PCR as a Simple and Efficient Method for Detection of Brucella spp. Infection

Alireza Moradabadi 1 , Azam Ahmadi 1 , Manijeh Kahbazi 1 , * , Hossein Sarmadian 1 and Mohammad Arjomandzadegan 1
Authors Information
1 Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
Article information
  • Jundishapur Journal of Microbiology: In Press (In Press); e14463
  • Published Online: April 9, 2019
  • Article Type: Research Article
  • Received: June 2, 2017
  • Revised: February 17, 2019
  • Accepted: February 25, 2019
  • DOI: 10.5812/jjm.14463

To Cite: Moradabadi A , Ahmadi A , Kahbazi M, Sarmadian H, Arjomandzadegan M. FLASH-PCR as a Simple and Efficient Method for Detection of Brucella spp. Infection, Jundishapur J Microbiol. Online ahead of Print ; In Press(In Press):e14463. doi: 10.5812/jjm.14463.

Abstract
Copyright © 2019, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Elfaki MG, Uz-Zaman T, Al-Hokail AA, Nakeeb SM. Detection of Brucella DNA in sera from patients with brucellosis by polymerase chain reaction. Diagn Microbiol Infect Dis. 2005;53(1):1-7. doi: 10.1016/j.diagmicrobio.2005.03.011. [PubMed: 16054326].
  • 2. Smith LD, Ficht TA. Pathogenesis of Brucella. Crit Rev Microbiol. 1990;17(3):209-30. doi: 10.3109/10408419009105726. [PubMed: 2154232].
  • 3. Meyer M. Current concepts in the taxonomy of the genus Brucella. Animal brucellosis. 1990:1-17.
  • 4. Davies CR. Zoonoses. Eds. S. R. Palmer, Lord Soulsby and D. I. H. Simpson. Oxford Medical Publications 1998. Pp. 640; 250 photographs, 100 figs. Epidemiol Infect. 1999;122(2):347-50. doi: 10.1017/s0950268899231986.
  • 5. Jansen W, Linard C, Noll M, Nöckler K, Al Dahouk S. Brucella-positive raw milk cheese sold on the inner European market: A public health threat due to illegal import? Food Control. 2019;100:130-7. doi: 10.1016/j.foodcont.2019.01.022.
  • 6. Taherahmadi H, Moradabadi AR, Nazari J, Kahbazi M. Antibiotic induced hemolytic anemia and thrombocytopenia among pediatric patients admitted to intensive care unit. Iran J Pediatr Hematol Oncol. 2019;9(1):9-16.
  • 7. Young EJ. An overview of human brucellosis. Clin Infect Dis. 1995;21(2):283-9. quiz 290. [PubMed: 8562733].
  • 8. Alton GG, Jones LM, Pietz DE. Laboratory techniques in brucellosis. World Health Organization; 1975.
  • 9. Staszkiewicz J, Lewis CM, Colville J, Zervos M, Band J. Outbreak of Brucella melitensis among microbiology laboratory workers in a community hospital. J Clin Microbiol. 1991;29(2):287-90. [PubMed: 2007637]. [PubMed Central: PMC269755].
  • 10. Al-Sekait MA. Seroepidemiology survey of brucellosis antibodies in Saudi Arabia. Ann Saudi Med. 1999;19(3):219-22. [PubMed: 17283457].
  • 11. Baily GG, Krahn JB, Drasar BS, Stoker NG. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg. 1992;95(4):271-5. [PubMed: 1495123].
  • 12. Herman L, De Ridder H. Identification of Brucella spp. by using the polymerase chain reaction. Appl Environ Microbiol. 1992;58(6):2099-101. [PubMed: 1377903]. [PubMed Central: PMC195734].
  • 13. Matar GM, Khneisser IA, Abdelnoor AM. Rapid laboratory confirmation of human brucellosis by PCR analysis of a target sequence on the 31-kilodalton Brucella antigen DNA. J Clin Microbiol. 1996;34(2):477-8. [PubMed: 8789045]. [PubMed Central: PMC228827].
  • 14. Morata P, Queipo-Ortuno MI, de Dios Colmenero J. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol. 1998;36(9):2443-6. [PubMed: 9705371]. [PubMed Central: PMC105141].
  • 15. Abramova SL, Ryazantsev DY, Voinova TM, Zavriev SK. Diagnostics of phytopathogen fungi Septoria tritici and Stagonospora nodorum by fluorescent amplification-based specific hybridization (FLASH) PCR. Rus J Bioorgan Chem. 2011;34(1):97-102. doi: 10.1134/s1068162008010135.
  • 16. Beck J. Polymerase chain reaction assays for the detection of Stagonospora nodorum and Septoria tritici in wheat. Phytopathol. 1995;85(3):319. doi: 10.1094/Phyto-85-319.
  • 17. Newby DT, Hadfield TL, Roberto FF. Real-time PCR detection of Brucella abortus: A comparative study of SYBR green I, 5'-exonuclease, and hybridization probe assays. Appl Environ Microbiol. 2003;69(8):4753-9. [PubMed: 12902268]. [PubMed Central: PMC169142].
  • 18. Moradabadi A, Farsinezhad A, Fekri Soofiabadi M. [Fast method for diagnosis of leishmania by PCR and FLASH PCR]. J Arak Univ Med Sci. 2017;19(11):79-86. Persian.
  • 19. Morad Abadi AR, Arjomandzadegan M, Emami N, Kahbazi M, Ahmadi A, Falahat S, et al. [Comparison of detection of clinical isolates of mycobacterium tuberculosis by flash PCR and conventional culture method]. J Arak Univ Med Sci. 2018;21(4):77-85. Persian.
  • 20. Cunfer BM, Ueng PP. Taxonomy and identification of septoria and stagonospora species on small-grain cereals. Annu Rev Phytopathol. 1999;37:267-84. doi: 10.1146/annurev.phyto.37.1.267. [PubMed: 11701824].
  • 21. Khatami F, Heidari M, Khatami M. Rapid detection of Escherichia coli O157: H7 by fluorescent amplification-based specific hybridization (FLASH) PCR. Iran Red Crescent Med J. 2012;14(9):594-8. [PubMed: 23115724]. [PubMed Central: PMC3482334].
  • 22. Elfaki MG, Al-Hokail AA, Nakeeb SM, Al-Rabiah FA. Evaluation of culture, tube agglutination, and PCR methods for the diagnosis of brucellosis in humans. Med Sci Monit. 2005;11(11):MT69-74. [PubMed: 16258407].
  • 23. Fekete A, Bantle JA, Halling SM, Sanborn MR. Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. J Appl Bacteriol. 1990;69(2):216-27. [PubMed: 2272943].
  • 24. Zerva L, Bourantas K, Mitka S, Kansouzidou A, Legakis NJ. Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. J Clin Microbiol. 2001;39(4):1661-4. doi: 10.1128/JCM.39.4.1661-1664.2001. [PubMed: 11283112]. [PubMed Central: PMC87995].
  • 25. Probert WS, Schrader KN, Khuong NY, Bystrom SL, Graves MH. Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J Clin Microbiol. 2004;42(3):1290-3. [PubMed: 15004098]. [PubMed Central: PMC356861].

Featured Image:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments