Jundishapur Journal of Microbiology

Published by: Kowsar

The Effect of Adding Blood on the Virulence Genes Expression of Staphylococcus aureus in Exponential and Stationary Growth Phase

Hadi Koohsari 1 , Ezzat Allah Ghaemi 2 , * , Noor Amir Mozaffari 3 , Abdolvahhab Moradi 2 , Maryam Sadegh-Sheshpoli 4 and Seyyed-Naemeh Javid 2
Authors Information
1 Department of Microbiology, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran
2 Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran
3 Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran
4 Department of Molecular Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Article information
  • Jundishapur Journal of Microbiology: June 2017, 10 (6); e14380
  • Published Online: June 12, 2017
  • Article Type: Research Article
  • Received: April 19, 2016
  • Revised: April 23, 2017
  • Accepted: May 26, 2017
  • DOI: 10.5812/jjm.14380

To Cite: Koohsari H, Ghaemi E A, Mozaffari N A, Moradi A, Sadegh-Sheshpoli M, et al. The Effect of Adding Blood on the Virulence Genes Expression of Staphylococcus aureus in Exponential and Stationary Growth Phase, Jundishapur J Microbiol. 2017 ; 10(6):e14380. doi: 10.5812/jjm.14380.

Copyright © 2017, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Bien J, Sokolova O, Bozko P. Characterization of Virulence Factors of Staphylococcus aureus: Novel Function of Known Virulence Factors That Are Implicated in Activation of Airway Epithelial Proinflammatory Response. J Pathog. 2011; 2011: 601905[DOI][PubMed]
  • 2. Costa AR, Batistão DWF, Ribas RM, Sousa AM, Pereira MO, Botelho CM. Microbial pathogens and strategies for combating them: science, technology and education. 2013; 1: 702-10
  • 3. Gray B, Hall P, Gresham H. Targeting agr- and agr-Like quorum sensing systems for development of common therapeutics to treat multiple gram-positive bacterial infections. Sensors (Basel). 2013; 13(4): 5130-66[DOI][PubMed]
  • 4. Wang B, Muir TW. Regulation of Virulence in Staphylococcus aureus: Molecular Mechanisms and Remaining Puzzles. Cell Chem Biol. 2016; 23(2): 214-24[DOI][PubMed]
  • 5. Oogai Y, Matsuo M, Hashimoto M, Kato F, Sugai M, Komatsuzawa H. Expression of virulence factors by Staphylococcus aureus grown in serum. Appl Environ Microbiol. 2011; 77(22): 8097-105[DOI][PubMed]
  • 6. Willey J, Sherwood L, Woolverton C. Prescott’s Microbiology. 2008; : 119-48
  • 7. Koohsari H, Mozafari NA, Ghazi-Saidi K, Nejad RAK, Moradi A. Effect of blood and sera on growth of staphylococcus aureus in BHI media. J Pure Appl Microbiol. 2013; 7(4): 2851-5
  • 8. Qiu J, Zhang X, Luo M, Li H, Dong J, Wang J, et al. Subinhibitory concentrations of perilla oil affect the expression of secreted virulence factor genes in Staphylococcus aureus. PLoS One. 2011; 6(1)[DOI][PubMed]
  • 9. Loughman JA, Fritz SA, Storch GA, Hunstad DA. Virulence gene expression in human community‐acquiredstaphylococcus aureusinfection. J Infect Dis. 2009; 199(3): 294-301[DOI]
  • 10. Mehrotra M, Wang G, Johnson WM. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol. 2000; 38(3): 1032-5[PubMed]
  • 11. Seidl K, Chen L, Bayer AS, Hady WA, Kreiswirth BN, Xiong YQ. Relationship of agr expression and function with virulence and vancomycin treatment outcomes in experimental endocarditis due to methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2011; 55(12): 5631-9[DOI][PubMed]
  • 12. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR. Basic principles of real-time quantitative PCR. Expert Rev Mol Diagn. 2005; 5(2): 209-19[DOI][PubMed]
  • 13. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001; 25(4): 402-8[DOI][PubMed]
  • 14. Malachowa N, Whitney AR, Kobayashi SD, Sturdevant DE, Kennedy AD, Braughton KR, et al. Global changes in Staphylococcus aureus gene expression in human blood. PLoS One. 2011; 6(4)[DOI][PubMed]
  • 15. Wiltshire MD, Foster SJ. Identification and analysis of Staphylococcus aureus components expressed by a model system of growth in serum. Infect Immun. 2001; 69(8): 5198-202[DOI][PubMed]
  • 16. Antunes LC, Ferreira RB, Buckner MM, Finlay BB. Quorum sensing in bacterial virulence. Microbiology. 2010; 156: 2271-82[DOI][PubMed]
  • 17. Dunman PM, Murphy E, Haney S, Palacios D, Tucker-Kellogg G, Wu S, et al. Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci. J Bacteriol. 2001; 183(24): 7341-53[DOI][PubMed]
  • 18. Novick RP, Geisinger E. Quorum sensing in staphylococci. Annu Rev Genet. 2008; 42: 541-64[DOI][PubMed]
  • 19. Burnside K, Lembo A, de Los Reyes M, Iliuk A, Binhtran NT, Connelly JE, et al. Regulation of hemolysin expression and virulence of Staphylococcus aureus by a serine/threonine kinase and phosphatase. PLoS One. 2010; 5(6)[DOI][PubMed]
  • 20. Mullarky IK, Su C, Frieze N, Park YH, Sordillo LM. Staphylococcus aureus agr genotypes with enterotoxin production capabilities can resist neutrophil bactericidal activity. Infect Immun. 2001; 69(1): 45-51[DOI][PubMed]
  • 21. Papakyriacou H, Vaz D, Simor A, Louie M, McGavin MJ. Molecular analysis of the accessory gene regulator (agr) locus and balance of virulence factor expression in epidemic methicillin-resistant Staphylococcus aureus. J Infect Dis. 2000; 181(3): 990-1000[DOI][PubMed]
  • 22. Cheung GY, Wang R, Khan BA, Sturdevant DE, Otto M. Role of the accessory gene regulator agr in community-associated methicillin-resistant Staphylococcus aureus pathogenesis. Infect Immun. 2011; 79(5): 1927-35[DOI][PubMed]
  • 23. O'Donnell S, Humphreys H, Hughes D. Distribution of virulence genes among colonising and invasive isolates of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect. 2008; 14(6): 625-6[DOI]
  • 24. Rasmussen G, Monecke S, Ehricht R, Soderquist B. Prevalence of clonal complexes and virulence genes among commensal and invasive Staphylococcus aureus isolates in Sweden. PLoS One. 2013; 8(10)[DOI][PubMed]
  • 25. Abu Othman A, Humphreys H, O'Neill E, Fitzgerald-Hughes D. Differences in expression of virulence genes amongst invasive and colonizing isolates of meticillin-resistant Staphylococcus aureus. J Med Microbiol. 2011; 60: 259-61[DOI][PubMed]
  • 26. Collins J, Rudkin J, Recker M, Pozzi C, O'Gara JP, Massey RC. Offsetting virulence and antibiotic resistance costs by MRSA. ISME J. 2010; 4(4): 577-84[DOI][PubMed]
  • 27. Jimenez JN, Ocampo AM, Vanegas JM, Rodriguez EA, Garces CG, Patino LA, et al. Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellin, Colombia. Mem Inst Oswaldo Cruz. 2011; 106(8): 980-5[DOI][PubMed]
  • 28. Otto M. Basis of virulence in community-associated methicillin-resistant Staphylococcus aureus. Annu Rev Microbiol. 2010; 64: 143-62[DOI][PubMed]
  • 29. Lee SM, Ender M, Adhikari R, Smith JM, Berger-Bachi B, Cook GM. Fitness cost of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus by way of continuous culture. Antimicrob Agents Chemother. 2007; 51(4): 1497-9[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments