Jundishapur Journal of Microbiology

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Molecular Epidemiology of Clonally Related Metallo-β-Lactamase-Producing Klebsiella pneumoniae Isolated from Newborns in a Hospital in Shandong, China

Yan Jin 1 , Chunzhong Dong 1 , Chunhong Shao 1 , Yong Wang 1 and Yun Liu 1 , *
Authors Information
1 Clinical Laboratory of Provincial Hospital affiliated with Shandong University, Jinan, Shandong, China
Article information
  • Jundishapur Journal of Microbiology: September 2017, 10 (9); e14046
  • Published Online: September 2, 2017
  • Article Type: Research Article
  • Received: December 24, 2016
  • Revised: May 25, 2017
  • Accepted: June 24, 2017
  • DOI: 10.5812/jjm.14046

To Cite: Jin Y, Dong C, Shao C, Wang Y, Liu Y. Molecular Epidemiology of Clonally Related Metallo-β-Lactamase-Producing Klebsiella pneumoniae Isolated from Newborns in a Hospital in Shandong, China, Jundishapur J Microbiol. 2017 ; 10(9):e14046. doi: 10.5812/jjm.14046.

Copyright © 2017, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Bennet R, Eriksson M, Zetterstrom R. Bacterial etiology of neonatal septicemia in relation to prior antibiotic treatment. Acta Paediatr Scand. 1987; 76(4): 673-4[DOI][PubMed]
  • 2. Zhou J, Li G, Ma X, Yang Q, Yi J. Outbreak of colonization by carbapenemase-producing Klebsiella pneumoniae in a neonatal intensive care unit: Investigation, control measures and assessment. Am J Infect Control. 2015; 43(10): 1122-4[DOI][PubMed]
  • 3. Ruppe E, Woerther PL, Barbier F. Mechanisms of antimicrobial resistance in Gram-negative bacilli. Ann Intensive Care. 2015; 5(1): 61[DOI][PubMed]
  • 4. Morrill HJ, Pogue JM, Kaye KS, LaPlante KL. Treatment Options for Carbapenem-Resistant Enterobacteriaceae Infections. Open Forum Infect Dis. 2015; 2(2)[DOI][PubMed]
  • 5. Queenan AM, Bush K. Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev. 2007; 20(3): 440-58[DOI][PubMed]
  • 6. Ambler RP. The structure of beta-lactamases. Philos Trans R Soc Lond B Biol Sci. 1980; 289(1036): 321-31[PubMed]
  • 7. Logan LK. Carbapenem-resistant enterobacteriaceae: an emerging problem in children. Clin Infect Dis. 2012; 55(6): 852-9[DOI][PubMed]
  • 8. Bushnell G, Mitrani-Gold F, Mundy LM. Emergence of New Delhi metallo-beta-lactamase type 1-producing enterobacteriaceae and non-enterobacteriaceae: global case detection and bacterial surveillance. Int J Infect Dis. 2013; 17(5)-33[DOI][PubMed]
  • 9. Escobar Perez JA, Olarte Escobar NM, Castro-Cardozo B, Valderrama Marquez IA, Garzon Aguilar MI, Martinez de la Barrera L, et al. Outbreak of NDM-1-producing Klebsiella pneumoniae in a neonatal unit in Colombia. Antimicrob Agents Chemother. 2013; 57(4): 1957-60[DOI][PubMed]
  • 10. Giuffre M, Bonura C, Geraci DM, Saporito L, Catalano R, Di Noto S, et al. Successful control of an outbreak of colonization by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae sequence type 258 in a neonatal intensive care unit, Italy. J Hosp Infect. 2013; 85(3): 233-6[DOI][PubMed]
  • 11. Yu F, Ying Q, Chen C, Li T, Ding B, Liu Y, et al. Outbreak of pulmonary infection caused by Klebsiella pneumoniae isolates harbouring blaIMP-4 and blaDHA-1 in a neonatal intensive care unit in China. J Med Microbiol. 2012; 61: 984-9[DOI][PubMed]
  • 12. Lee K, Chong Y, Shin HB, Kim YA, Yong D, Yum JH. Modified Hodge and EDTA-disk synergy tests to screen metallo-beta-lactamase-producing strains of Pseudomonas and Acinetobacter species. Clin Microbiol Infect. 2001; 7(2): 88-91[PubMed]
  • 13. Aubron C, Poirel L, Ash RJ, Nordmann P. Carbapenemase-producing Enterobacteriaceae, U.S. rivers. Emerg Infect Dis. 2005; 11(2): 260-4[DOI][PubMed]
  • 14. Bradford PA, Bratu S, Urban C, Visalli M, Mariano N, Landman D, et al. Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 beta-lactamases in New York City. Clin Infect Dis. 2004; 39(1): 55-60[DOI][PubMed]
  • 15. Dallenne C, Da Costa A, Decre D, Favier C, Arlet G. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. J Antimicrob Chemother. 2010; 65(3): 490-5[DOI][PubMed]
  • 16. Queenan AM, Torres-Viera C, Gold HS, Carmeli Y, Eliopoulos GM, Moellering RJ, et al. SME-type carbapenem-hydrolyzing class A beta-lactamases from geographically diverse Serratia marcescens strains. Antimicrob Agents Chemother. 2000; 44(11): 3035-9[PubMed]
  • 17. Radice M, Power P, Gutkind G, Fernandez K, Vay C, Famiglietti A, et al. First class a carbapenemase isolated from enterobacteriaceae in Argentina. Antimicrob Agents Chemother. 2004; 48(3): 1068-9[DOI][PubMed]
  • 18. Tijet N, Alexander DC, Richardson D, Lastovetska O, Low DE, Patel SN, et al. New Delhi metallo-beta-lactamase, Ontario, Canada. Emerg Infect Dis. 2011; 17(2): 306-7[DOI][PubMed]
  • 19. Zhao WH, Chen G, Ito R, Hu ZQ. Relevance of resistance levels to carbapenems and integron-borne blaIMP-1, blaIMP-7, blaIMP-10 and blaVIM-2 in clinical isolates of Pseudomonas aeruginosa. J Med Microbiol. 2009; 58: 1080-5[DOI][PubMed]
  • 20. Barton BM, Harding GP, Zuccarelli AJ. A general method for detecting and sizing large plasmids. Anal Biochem. 1995; 226(2): 235-40[DOI][PubMed]
  • 21. Goldstein C, Lee MD, Sanchez S, Hudson C, Phillips B, Register B, et al. Incidence of class 1 and 2 integrases in clinical and commensal bacteria from livestock, companion animals, and exotics. Antimicrob Agents Chemother. 2001; 45(3): 723-6[DOI][PubMed]
  • 22. Levesque C, Piche L, Larose C, Roy PH. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother. 1995; 39(1): 185-91[DOI][PubMed]
  • 23. Maslow JN, Brecher SM, Adams KS, Durbin A, Loring S, Arbeit RD. Relationship between indole production and differentiation of Klebsiella species: indole-positive and -negative isolates of Klebsiella determined to be clonal. J Clin Microbiol. 1993; 31(8): 2000-3[PubMed]
  • 24. Hunter SB, Vauterin P, Lambert-Fair MA, Van Duyne MS, Kubota K, Graves L, et al. Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. J Clin Microbiol. 2005; 43(3): 1045-50[DOI][PubMed]
  • 25. Calfee D, Jenkins SG. Use of active surveillance cultures to detect asymptomatic colonization with carbapenem-resistant Klebsiella pneumoniae in intensive care unit patients. Infect Control Hosp Epidemiol. 2008; 29(10): 966-8[DOI][PubMed]
  • 26. Centers for Disease C. Carbapenem-resistant Enterobacteriaceae containing New Delhi metallo-beta-lactamase in two patients - Rhode Island, March 2012. MMWR Morb Mortal Wkly Rep. 2012; 61(24): 446-8[PubMed]
  • 27. Gaibani P, Ambretti S, Berlingeri A, Cordovana M, Farruggia P, Panico M, et al. Outbreak of NDM-1-producing Enterobacteriaceae in northern Italy, July to August 2011. Euro Surveill. 2011; 16(47): 20027[PubMed]
  • 28. Roy S, Singh AK, Viswanathan R, Nandy RK, Basu S. Transmission of imipenem resistance determinants during the course of an outbreak of NDM-1 Escherichia coli in a sick newborn care unit. J Antimicrob Chemother. 2011; 66(12): 2773-80[DOI][PubMed]
  • 29. Borer A, Saidel-Odes L, Riesenberg K, Eskira S, Peled N, Nativ R, et al. Attributable mortality rate for carbapenem-resistant Klebsiella pneumoniae bacteremia. Infect Control Hosp Epidemiol. 2009; 30(10): 972-6[DOI][PubMed]
  • 30. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, et al. Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother. 2009; 53(12): 5046-54[DOI][PubMed]
  • 31. Wang P, Hu F, Xiong Z, Ye X, Zhu D, Wang YF, et al. Susceptibility of extended-spectrum-beta-lactamase-producing Enterobacteriaceae according to the new CLSI breakpoints. J Clin Microbiol. 2011; 49(9): 3127-31[DOI][PubMed]
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