Jundishapur Journal of Microbiology

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Clinical Isolates of Acinetobacter baumannii From Tehran Hospitals: Pulsed-field Gel Electrophoresis Characterization, Clonal Lineages, Antibiotic Susceptibility, and Biofilm-forming Ability

Mahdi Akbari Dehbalaei 1 , Shahin Najar-Peerayeh 1 , * , Morovat Taherikalani 2 and Mehrdad Behmanesh 3
Authors Information
1 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2 Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
3 Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: July 2017, 10 (7); e13790
  • Published Online: May 31, 2017
  • Article Type: Research Article
  • Received: January 3, 2017
  • Revised: March 9, 2017
  • Accepted: March 18, 2017
  • DOI: 10.5812/jjm.13790

To Cite: Akbari Dehbalaei M, Najar-Peerayeh S, Taherikalani M, Behmanesh M. Clinical Isolates of Acinetobacter baumannii From Tehran Hospitals: Pulsed-field Gel Electrophoresis Characterization, Clonal Lineages, Antibiotic Susceptibility, and Biofilm-forming Ability, Jundishapur J Microbiol. 2017 ; 10(7):e13790. doi: 10.5812/jjm.13790.

Copyright © 2017, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev. 2008; 21(3): 538-82[DOI][PubMed]
  • 2. Seifert H, Schulze A, Baginski R, Pulverer G. Comparison of four different methods for epidemiologic typing of Acinetobacter baumannii. J Clin Microbiol. 1994; 32(7): 1816-9[PubMed]
  • 3. Hartstein AI, Morthland VH, Rourke JW, Freeman J, Garber S, Sykes R, et al. Plasmid DNA fingerprinting of Acinetobacter calcoaceticus subspecies anitratus from intubated and mechanically ventilated patients. Infect Control Hosp Epidemiol. 1990; 11(10): 531-8[PubMed]
  • 4. Seifert H, Baginski R, Schulze A, Pulverer G. Antimicrobial susceptibility of Acinetobacter species. Antimicrob Agents Chemother. 1993; 37(4): 750-3[PubMed]
  • 5. Maragakis LL, Perl TM. Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options. Clin Infect Dis. 2008; 46(8): 1254-63[DOI][PubMed]
  • 6. Zarrilli R, Giannouli M, Tomasone F, Triassi M, Tsakris A. Carbapenem resistance in Acinetobacter baumannii: the molecular epidemic features of an emerging problem in health care facilities. J Infect Dev Ctries. 2009; 3(5): 335-41[PubMed]
  • 7. Lin MF, Lan CY. Antimicrobial resistance in Acinetobacter baumannii: From bench to bedside. World J Clin Cases. 2014; 2(12): 787-814[DOI][PubMed]
  • 8. Villalon P, Valdezate S, Medina-Pascual MJ, Rubio V, Vindel A, Saez-Nieto JA. Clonal diversity of nosocomial epidemic Acinetobacter baumannii strains isolated in Spain. J Clin Microbiol. 2011; 49(3): 875-82[DOI][PubMed]
  • 9. Da Silva G, Dijkshoorn L, van der Reijden T, van Strijen B, Duarte A. Identification of widespread, closely related Acinetobacter baumannii isolates in Portugal as a subgroup of European clone II. Clin Microbiol Infect. 2007; 13(2): 190-5[DOI][PubMed]
  • 10. Di Popolo A, Giannouli M, Triassi M, Brisse S, Zarrilli R. Molecular epidemiological investigation of multidrug-resistant Acinetobacter baumannii strains in four Mediterranean countries with a multilocus sequence typing scheme. Clin Microbiol Infect. 2011; 17(2): 197-201[DOI][PubMed]
  • 11. Giannouli M, Cuccurullo S, Crivaro V, Di Popolo A, Bernardo M, Tomasone F, et al. Molecular epidemiology of multidrug-resistant Acinetobacter baumannii in a tertiary care hospital in Naples, Italy, shows the emergence of a novel epidemic clone. J Clin Microbiol. 2010; 48(4): 1223-30[DOI][PubMed]
  • 12. Turton JF, Woodford N, Glover J, Yarde S, Kaufmann ME, Pitt TL. Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J Clin Microbiol. 2006; 44(8): 2974-6[DOI][PubMed]
  • 13. Patel J, Cockerill F, Alder J, Bradford P, Eliopoulos G, Hardy D. Performance standards for antimicrobial susceptibility testing; twenty-fourth informational supplement. 2014; 34
  • 14. Abdalhamid B, Hassan H, Itbaileh A, Shorman M. Characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates in a tertiary care hospital in Saudi Arabia. New Microbiol. 2014; 37(1): 65-73[PubMed]
  • 15. Badmasti F, Siadat SD, Bouzari S, Ajdary S, Shahcheraghi F. Molecular detection of genes related to biofilm formation in multidrug-resistant Acinetobacter baumannii isolated from clinical settings. J Med Microbiol. 2015; 64: 559-64[DOI][PubMed]
  • 16. Durmaz R, Otlu B, Koksal F, Hosoglu S, Ozturk R, Ersoy Y, et al. The optimization of a rapid pulsed-field gel electrophoresis protocol for the typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp. Jpn J Infect Dis. 2009; 62(5): 372-7[PubMed]
  • 17. Turton JF, Gabriel SN, Valderrey C, Kaufmann ME, Pitt TL. Use of sequence-based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumannii. Clin Microbiol Infect. 2007; 13(8): 807-15[DOI][PubMed]
  • 18. Anvarinejad M, Japoni A, Davarpanah MA, Mahmudi H, Mammina C, Vazin A. Phenotypic and Molecular Epidemiology of Acinetobacter calcoaceticus baumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran. Jundishapur J Microbiol. 2015; 8(6): 19180[DOI][PubMed]
  • 19. Falagas ME, Karveli EA. The changing global epidemiology of Acinetobacter baumannii infections: a development with major public health implications. Clin Microbiol Infect. 2007; 13(2): 117-9[DOI][PubMed]
  • 20. Van Looveren M, Goossens H, Arpac Steering Group . Antimicrobial resistance of Acinetobacter spp. in Europe. Clin Microbiol Infect. 2004; 10(8): 684-704[DOI][PubMed]
  • 21. Hamouda A, Evans BA, Towner KJ, Amyes SG. Characterization of epidemiologically unrelated Acinetobacter baumannii isolates from four continents by use of multilocus sequence typing, pulsed-field gel electrophoresis, and sequence-based typing of bla(OXA-51-like) genes. J Clin Microbiol. 2010; 48(7): 2476-83[DOI][PubMed]
  • 22. Shelburne S3, Singh KV, White AC, Byrne L, Carmer A, Austin C, et al. Sequential outbreaks of infections by distinct Acinetobacter baumannii strains in a public teaching hospital in Houston, Texas. J Clin Microbiol. 2008; 46(1): 198-205[DOI][PubMed]
  • 23. Mohajeri P, Farahani A, Feizabadi MM, Ketabi H, Abiri R, Najafi F. Antimicrobial susceptibility profiling and genomic diversity of Acinetobacter baumannii isolates: A study in western Iran. Iran J Microbiol. 2013; 5(3): 195-202[PubMed]
  • 24. Shahcheraghi F, Abbasalipour M, Feizabadi M, Ebrahimipour G, Akbari N. Isolation and genetic characterization of metallo-beta-lactamase and carbapenamase producing strains of Acinetobacter baumannii from patients at Tehran hospitals. Iran J Microbiol. 2011; 3(2): 68-74[PubMed]
  • 25. Najar Peerayeh S, Karmostaji A. Molecular Identification of Resistance Determinants, Integrons and Genetic Relatedness of Extensively Drug Resistant Acinetobacter baumannii Isolated From Hospitals in Tehran, Iran. Jundishapur J Microbiol. 2015; 8(7): 27021[DOI][PubMed]
  • 26. Bahador A, Taheri M, Pourakbari B, Hashemizadeh Z, Rostami H, Mansoori N, et al. Emergence of rifampicin, tigecycline, and colistin-resistant Acinetobacter baumannii in Iran; spreading of MDR strains of novel International Clone variants. Microb Drug Resist. 2013; 19(5): 397-406[DOI][PubMed]
  • 27. Mahdian S, Sadeghifard N, Pakzad I, Ghanbari F, Soroush S, Azimi L, et al. Acinetobacter baumannii clonal lineages I and II harboring different carbapenem-hydrolyzing-beta-lactamase genes are widespread among hospitalized burn patients in Tehran. J Infect Public Health. 2015; 8(6): 533-42[DOI][PubMed]
  • 28. Mendes RE, Farrell DJ, Sader HS, Jones RN. Comprehensive assessment of tigecycline activity tested against a worldwide collection of Acinetobacter spp. (2005-2009). Diagn Microbiol Infect Dis. 2010; 68(3): 307-11[DOI][PubMed]
  • 29. Moradi J, Hashemi FB, Bahador A. Antibiotic Resistance of Acinetobacter baumannii in Iran: A Systemic Review of the Published Literature. Osong Public Health Res Perspect. 2015; 6(2): 79-86[DOI][PubMed]
  • 30. Karmostaj A, Najar-Peerayeh S, Salmanian AH. Emergence of tigecycline resistant Acinetobacter baumannii from an intensive care unit (ICU) in Tehran. Jundishapur J Microbiol. 2013; 6(3): 215-9[DOI]
  • 31. Karmostaji A, Najar-Peerayeh S, Salmanian AH. Distribution of OXA-type class D β-lactamase genes among nosocomial multi drug resistant Acinetobacter baumannii isolated in Tehran hospitals. Jundishapur J Microbiol. 2013; 6(5)[DOI]
  • 32. Bahador A, Raoo An R, Farshadzadeh Z, Beitollahi L, Khaledi A, Rahimi S, et al. The Prevalence of IS Aba 1 and IS Aba 4 in Acinetobacter baumannii Species of Different International Clone Lineages Among Patients With Burning in Tehran, Iran. Jundishapur J Microbiol. 2015; 8(7): 17167[DOI][PubMed]
  • 33. Kaliterna V, Kaliterna M, Hrenovic J, Barisic Z, Tonkic M, Goic-Barisic I. Acinetobacter baumannii in Southern Croatia: clonal lineages, biofilm formation, and resistance patterns. Infect Dis (Lond). 2015; 47(12): 902-7[DOI][PubMed]
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