Jundishapur Journal of Microbiology

Published by: Kowsar

Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran

Seyed Asghar Havaei 1 , 2 , Mehrdad Halaji 1 , 2 , Sinisa Vidovic 3 , Jo-Anne R. Dillon 3 , Mohsen Karbalaei 4 , Fahimeh Ghanbari 5 , Amirmorteza Ebrahimzadeh Namvar 6 , Bahram Nasr Esfahani 1 , Seyed Mehdi Havaei 7 and Amir Azimian 8 , *
Authors Information
1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Canada
4 Antimicrobial Resistance Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5 Department of Microbiology, Faculty of Bioscience, Islamic Azad University, Falavarjan Branch, Isfahan, Iran
6 Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
7 Department of Biology, Faculty of Sciences, Shiraz University, Shiraz, Iran
8 Department of Pathobiology and Laboratory Sciences, North Khorasan University of Medical Sciences, Bojnurd, Iran
Article information
  • Jundishapur Journal of Microbiology: May 2017, 10 (5); e13571
  • Published Online: May 17, 2017
  • Article Type: Research Article
  • Received: November 21, 2016
  • Revised: March 21, 2017
  • Accepted: April 15, 2017
  • DOI: 10.5812/jjm.13571

To Cite: Havaei S A, Halaji M, Vidovic S, R. Dillon J, Karbalaei M, et al. Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran, Jundishapur J Microbiol. 2017 ; 10(5):e13571. doi: 10.5812/jjm.13571.

Copyright © 2017, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Havaei SA, Vidovic S, Tahmineh N, Mohammad K, Mohsen K, Starnino S, et al. Epidemic methicillin-susceptible Staphylococcus aureus lineages are the main cause of infections at an Iranian university hospital. J Clin Microbiol. 2011; 49(11): 3990-3[DOI][PubMed]
  • 2. Jevons MP. "Celbenin"-resistant staphylococci. British Med J. 1961; 1(5219): 124
  • 3. Stewart GT, Holt RJ. Evolutio of natural resistance to the newer penicillins. Br Med J. 1963; 1(5326): 308-11[PubMed]
  • 4. Cheng H, Yuan W, Zeng F, Hu Q, Shang W, Tang D, et al. Molecular and phenotypic evidence for the spread of three major methicillin-resistant Staphylococcus aureus clones associated with two characteristic antimicrobial resistance profiles in China. J Antimicrob Chemother. 2013; 68(11): 2453-7[DOI][PubMed]
  • 5. Wang WY, Chiueh TS, Sun JR, Tsao SM, Lu JJ. Molecular typing and phenotype characterization of methicillin-resistant Staphylococcus aureus isolates from blood in Taiwan. PLoS One. 2012; 7(1): 30394[DOI][PubMed]
  • 6. Chen CJ, Huang YC. New epidemiology of Staphylococcus aureus infection in Asia. Clin Microbiol Infect. 2014; 20(7): 605-23[DOI][PubMed]
  • 7. den Heijer CD, van Bijnen EM, Paget WJ, Pringle M, Goossens H, Bruggeman CA, et al. Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study. Lancet Infect Dis. 2013; 13(5): 409-15[DOI][PubMed]
  • 8. Rosenthal VD, Al-Abdely HM, El-Kholy AA, AlKhawaja SA, Leblebicioglu H, Mehta Y, et al. International Nosocomial Infection Control Consortium report, data summary of 50 countries for 2010-2015: Device-associated module. Am J Infect Control. 2016; 44(12): 1495-504[DOI][PubMed]
  • 9. Askari E, Soleymani F, Arianpoor A, Tabatabai SM, Amini A, Naderinasab M. Epidemiology of mecA-Methicillin Resistant Staphylococcus aureus (MRSA) in Iran: A Systematic Review and Meta-analysis. Iran J Basic Med Sci. 2012; 15(5): 1010-9[PubMed]
  • 10. Ebrahim-Saraie HS, Motamedifar M, Sarvari J, Hoseini Alfatemi SM. Emergence of SCCmec Type I Obtained From Clinical Samples in Shiraz Teaching Hospitals, South-West of Iran. Jundishapur J Microbiol. 2015; 8(6)[DOI][PubMed]
  • 11. Bai AD, Showler A, Burry L, Steinberg M, Ricciuto DR, Fernandes T, et al. Impact of Infectious Disease Consultation on Quality of Care, Mortality, and Length of Stay in Staphylococcus aureus Bacteremia: Results From a Large Multicenter Cohort Study. Clin Infect Dis. 2015; 60(10): 1451-61[DOI][PubMed]
  • 12. Vandecasteele SJ, Boelaert JR, De Vriese AS. Staphylococcus aureus infections in hemodialysis: what a nephrologist should know. Clin J Am Soc Nephrol. 2009; 4(8): 1388-400[DOI][PubMed]
  • 13. Hookey JV, Edwards V, Cookson BD, Richardson JF. PCR-RFLP analysis of the coagulase gene of Staphylococcus aureus: application to the differentiation of epidemic and sporadic methicillin-resistant strains. J Hosp Infect. 1999; 42(3): 205-12[DOI][PubMed]
  • 14. CLSI (2015). . Performance standards for antimicrobial susceptibility testing; 25th informational supplement.
  • 15. Swenson JM, Spargo J, Tenover FC, Ferraro MJ. Optimal inoculation methods and quality control for the NCCLS oxacillin agar screen test for detection of oxacillin resistance in Staphylococcus aureus. J Clin Microbiol. 2001; 39(10): 3781-4[DOI][PubMed]
  • 16. Kumar R, Yadav B, Dev K. A simple protocol for DNA extraction from staphylococcus aureus. 2009;
  • 17. Vannuffel P, Laterre PF, Bouyer M, Gigi J, Vandercam B, Reynaert M, et al. Rapid and specific molecular identification of methicillin-resistant Staphylococcus aureus in endotracheal aspirates from mechanically ventilated patients. J Clin Microbiol. 1998; 36(8): 2366-8[PubMed]
  • 18. Raimundo O, Deighton M, Capstick J, Gerraty N. Molecular typing of Staphylococcus aureus of bovine origin by polymorphisms of the coagulase gene. Vet Microbiol. 1999; 66(4): 275-84[PubMed]
  • 19. Guler L, Ok U, Gunduz K, Gulcu Y, Hadimli HH. Antimicrobial susceptibility and coagulase gene typing of Staphylococcus aureus isolated from bovine clinical mastitis cases in Turkey. J Dairy Sci. 2005; 88(9): 3149-54[DOI][PubMed]
  • 20. Boye K, Bartels MD, Andersen IS, Moller JA, Westh H. A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V. Clin Microbiol Infect. 2007; 13(7): 725-7[DOI][PubMed]
  • 21. Peerayeh SN, Azimian A, Nejad QB, Kashi M. Prevalence of agr specificity groups among Staphylococcus aureus isolates from university hospitals in Tehran. Laboratory Med. 2009; 40(1): 27-9[DOI]
  • 22. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol. 2000; 38(3): 1008-15[PubMed]
  • 23. Zeinali Nia N, Pourmand MR, Afrough P. Comparison of hypervariable region (hvr) of meca gene in staphylococcus aureus isolated from nasal carriers and clinical samples. Jundishapur J Microbiol. 2013; 6(9)[DOI]
  • 24. Mohajeri P, Azizkhani S, Farahani A, Norozi B. Genotyping of coa and aroA Genes of Methicillin-Resistant Staphylococcus aureus Strains Isolated From Nasal Samples in Western Iran. Jundishapur J Microbiol. 2016; 9(1)[DOI][PubMed]
  • 25. Foad Mirkarimi S, Hasani A, Abdinia B, Soroush Barhaghi MH, Nikbakht M, Ahangarzadeh Rezaee M. High diversity of methicillin-resistant staphylococcus aureus (mrsa) isolates based on hypervariable region polymorphisms. Arch Pediatr Infect Dis. 2016; 4(4)[DOI]
  • 26. Perez LR, Dias C, d'Azevedo PA. Agar dilution and agar screen with cefoxitin and oxacillin: what is known and what is unknown in detection of meticillin-resistant Staphylococcus aureus. J Med Microbiol. 2008; 57: 954-6[DOI][PubMed]
  • 27. Hallin M, Denis O, Deplano A, De Mendonca R, De Ryck R, Rottiers S, et al. Genetic relatedness between methicillin-susceptible and methicillin-resistant Staphylococcus aureus: results of a national survey. J Antimicrob Chemother. 2007; 59(3): 465-72[DOI][PubMed]
  • 28. Soge OO, Meschke JS, No DB, Roberts MC. Characterization of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches. J Antimicrob Chemother. 2009; 64(6): 1148-55[DOI][PubMed]
  • 29. Kim ES, Song JS, Lee HJ, Choe PG, Park KH, Cho JH, et al. A survey of community-associated methicillin-resistant Staphylococcus aureus in Korea. J Antimicrob Chemother. 2007; 60(5): 1108-14[DOI][PubMed]
  • 30. Himabindu M, Muthamilselvan DS, Bishi DK, Verma RS. Molecular analysis of coagulase gene polymorphism in clinical isolates of methicillin resistant Staphylococcus aureus by restriction fragment length polymorphism based genotyping. Am J Infect Dis. 2009; 5: 170-6
  • 31. Hata E, Katsuda K, Kobayashi H, Uchida I, Tanaka K, Eguchi M. Genetic variation among Staphylococcus aureus strains from bovine milk and their relevance to methicillin-resistant isolates from humans. J Clin Microbiol. 2010; 48(6): 2130-9[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments