Jundishapur Journal of Microbiology

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Molecular Identification of Sarcocystis spp. in Sheep and Cattle by PCR-RFLP from Southwest of Iran

Mahmoud Rahdar 1 , 2 , * and Tahereh Kardooni 2
Authors Information
1 Infectious and Tropical Diseases Research Center, Health Research Institute Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Department of Parasitology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: August 2017, 10 (8); e12798
  • Published Online: August 21, 2017
  • Article Type: Research Article
  • Received: May 9, 2017
  • Accepted: June 24, 2017
  • DOI: 10.5812/jjm.12798

To Cite: Rahdar M, Kardooni T. Molecular Identification of Sarcocystis spp. in Sheep and Cattle by PCR-RFLP from Southwest of Iran, Jundishapur J Microbiol. 2017 ; 10(8):e12798. doi: 10.5812/jjm.12798.

Abstract
Copyright © 2017, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Dubey JP, Speer CA, Fayer R. Sarcocystosis of animals and man. 1989;
  • 2. Tenter AM, Johnson AM. Phylogeny of the tissue cyst-forming coccidia. Adv Parasitol. 1997; 39: 69-139[PubMed]
  • 3. Fayer R, Esposito DH, Dubey JP. Human infections with Sarcocystis species. Clin Microbiol Rev. 2015; 28(2): 295-311[DOI][PubMed]
  • 4. Tenter AM. Current research on Sarcocystis species of domestic animals. Int J Parasitol. 1995; 25(11): 1311-30[PubMed]
  • 5. Soulsby EJL. Helminths, arthropods and protozoa of domesticated animals. 1982;
  • 6. Jeffrey M. Sarcocystosis of sheep. In Practice. 1993; 15(1): 2-8[DOI]
  • 7. Fayer R, Dubey JP. Sarcocystis induced abortion and fetal death. Prog Clin Biol Res. 1988; 281: 153-64[PubMed]
  • 8. O'Donoghue P, Rommel M. Australian-German collaborative studies on the immunology of Sarcocystis infections. Angew Parasitol. 1992; 33(2): 102-19[PubMed]
  • 9. Martínez-Navalon B, Anastasio-Giner B, Cano-Fructuoso M, Sanchez-Martínez P, Llopis-Morant A, Perez-Castarlenas B, et al. Sarcocystis infection: a major cause of carcass condemnation in adult sheep in Spain. Spanish J Agric Res. 2012; 10(2): 388-92
  • 10. Arness MK, Brown JD, Dubey JP, Neafie RC, Granstrom DE. An outbreak of acute eosinophilic myositis attributed to human Sarcocystis parasitism. Am J Trop Med Hyg. 1999; 61(4): 548-53[PubMed]
  • 11. Mehrotra R, Bisht D, Singh PA, Gupta SC, Gupta RK. Diagnosis of human sarcocystis infection from biopsies of the skeletal muscle. Pathology. 1996; 28(3): 281-2[PubMed]
  • 12. Tappe D, Abdullah S, Heo CC, Kannan Kutty M, Latif B. Human and animal invasive muscular sarcocystosis in Malaysia--recent cases, review and hypotheses. Trop Biomed. 2013; 30(3): 355-66[PubMed]
  • 13. Italiano CM, Wong KT, AbuBakar S, Lau YL, Ramli N, Omar SFS, et al. Sarcocystis nesbitti causes acute, relapsing febrile myositis with a high attack rate: description of a large outbreak of muscular sarcocystosis in Pangkor Island, Malaysia, 2012. PLoS Neglect Trop Dis. 2014; 8(5)
  • 14. Esposito DH, Stich A, Epelboin L, Malvy D, Han PV, Bottieau E, et al. Acute muscular sarcocystosis: an international investigation among ill travelers returning from Tioman Island, Malaysia, 2011–2012. Clin Infect Dis. 2014; 59(10): 1401-10[DOI][PubMed]
  • 15. Yang ZQ, Li QQ, Zuo YX, Chen XW, Chen YJ, Nie L, et al. Characterization of Sarcocystis species in domestic animals using a PCR-RFLP analysis of variation in the 18S rRNA gene: a cost-effective and simple technique for routine species identification. Exp Parasitol. 2002; 102(3-4): 212-7[PubMed]
  • 16. Yang ZQ, Zuo YX, Chen XW, Ding B, Luo J, Zhang YP. 18S rRNA gene of Sarcocystis hominis cyst from water buffalo and cattle. Zool Res. 2000; 21(2): 133-8
  • 17. Fischer S, Odening K. Characterization of bovine Sarcocystis species by analysis of their 18S ribosomal DNA sequences. J Parasitol. 1998; 84(1): 50-4[PubMed]
  • 18. Pritt B, Trainer T, Simmons-Arnold L, Evans M, Dunams D, Rosenthal BM. Detection of sarcocystis parasites in retail beef: a regional survey combining histological and genetic detection methods. J Food Prot. 2008; 71(10): 2144-7[PubMed]
  • 19. Rahdar M, Salehi M. The prevalence of sarcocystis infection in slaughtered cattle and sheep using digestion method in ahvaz city of iran. Biochem Cell Arch. 2011; 11(2): 469-71
  • 20. Wong KT, Pathmanathan R. Ultrastructure of the human skeletal muscle sarcocyst. J Parasitol. 1994; 80(2): 327-30[PubMed]
  • 21. McManus DP, Bowles J. Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. Int J Parasitol. 1996; 26(7): 687-704[PubMed]
  • 22. Agholi M, Shahabadi SN, Motazedian MH, Hatam GR. Prevalence of Enteric Protozoan Oocysts with Special Reference to Sarcocystis cruzi among Fecal Samples of Diarrheic Immunodeficient Patients in Iran. Korean J Parasitol. 2016; 54(3): 339-44[DOI][PubMed]
  • 23. Gjerde B. Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. Int J Parasitol. 2013; 43(7): 579-91[DOI][PubMed]
  • 24. More G, Pantchev A, Skuballa J, Langenmayer MC, Maksimov P, Conraths FJ, et al. Sarcocystis sinensis is the most prevalent thick-walled Sarcocystis species in beef on sale for consumers in Germany. Parasitol Res. 2014; 113(6): 2223-30[DOI][PubMed]
  • 25. Li QQ, Yang ZQ, Zuo YX, Attwood SW, Chen XW, Zhang YP. A PCR-based RFLP analysis of Sarcocystis cruzi (Protozoa: Sarcocystidae) in Yunnan Province, PR China, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host. J Parasitol. 2002; 88(6): 1259-61[DOI][PubMed]
  • 26. Jehle C, Dinkel A, Sander A, Morent M, Romig T, Luc PV, et al. Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam. Vet Parasitol. 2009; 166(3-4): 314-20[DOI][PubMed]
  • 27. Hajimohammadi B, Eslami G, Zohourtabar A, Dehghani A, Oryan A, Tafti H. High Occurrence of Sarcocystis Cysts in Meat Produced in Yazd, Central Iran. J Food Qual Hazards Control. 2014; 1(4): 95
  • 28. Nourollahi Fard SR, Asghari M, Nouri F. Survey of Sarcocystis infection in slaughtered cattle in Kerman, Iran. Trop Anim Health Prod. 2009; 41(8): 1633-6[DOI][PubMed]
  • 29. Nourollahi-Fard SR, Kheirandish R, Sattari S. Prevalence and histopathological finding of thin-walled and thick-walled Sarcocysts in slaughtered cattle of Karaj abattoir, Iran. J Parasit Dis. 2015; 39(2): 272-5[DOI][PubMed]
  • 30. Motamedi G, Dalimi A, Aghaeipour K, Nouri A. Ultrastructural and molecular studies on fat and thin macrocysts of Sarcocystis spp. isolated from naturally infected goats. Arch Razi Inst. 2010; 65(2): 91-7
  • 31. Bittencourt MV, Meneses ID, Ribeiro-Andrade M, de Jesus RF, de Araujo FR, Gondim LF. Sarcocystis spp. in sheep and goats: frequency of infection and species identification by morphological, ultrastructural, and molecular tests in Bahia, Brazil. Parasitol Res. 2016; 115(4): 1683-9[DOI][PubMed]
  • 32. Dubey JP, Lindsay DS, Speer CA, Fayer R, Livingston CJ. Sarcocystis arieticanis and other Sarcocystis species in sheep in the United States. J Parasitol. 1988; 74(6): 1033-8[PubMed]
  • 33. Farhang-Pajuh F, Yakhchali M, Mardani K. Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran. Vet Res Forum. 2014; 5(3): 181-6
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